Brea D. DuvalMindy G. ElrodJay E. GeeNarisara ChantratitaSarunporn TandhavanantDirek LimmathurotsakulAlex R. HoffmasterCenters for Disease Control and PreventionMahidol University2018-11-092018-11-092014-01-01American Journal of Tropical Medicine and Hygiene. Vol.90, No.6 (2014), 1043-1046000296372-s2.0-84902279551https://repository.li.mahidol.ac.th/handle/20.500.14594/34031Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested. Copyright © 2014 by The American Society of Tropical Medicine and Hygiene.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.4269/ajtmh.14-0025