Rattananinsruang P.Noonin C.Yoodee S.Thongboonkerd V.Mahidol University2023-05-192023-05-192023-01-01Environmental Toxicology and Pharmacology Vol.97 (2023)13826689https://repository.li.mahidol.ac.th/handle/20.500.14594/81893To address what marker(s) is/are most suitable for determining renal cell senescence, cell area, granularity, cycle shift/arrest, SA-β-Gal, SIRT1 and p16 were evaluated after inducing senescence in HK-2 cells with 0.2–0.8 mM H2O2. Only cell area and granularity concentration-dependently increased at all time-points, whereas SA-β-Gal, SIRT1 and p16 showed significant coefficient of determination (R2) at two time-points. Cell granularity had significant correlation coefficient (R) with other six, whereas SA-β-Gal had significant R with five, and cell area, SIRT1 and p16 had significant R with four others. Comparing to SA-β-Gal, other markers had significantly lower fold-changes only at 72-h with 0.8 mM H2O2, whereas p16 provided greater fold-changes at 48-h with 0.4 and 0.8 mM H2O2. Therefore, cell area, granularity, SA-β-Gal and p16 may serve as the most suitable markers for determining H2O2-induced senescence in HK-2 renal cells, whereas other markers can be also used but with inferior quantitative precision.Environmental ScienceArticleSCOPUS10.1016/j.etap.2022.1040392-s2.0-851444538531872707736528215