A. ApiluxY. UkitaM. ChikaeO. ChailapakulY. TakamuraJapan Advanced Institute of Science and TechnologyMahidol UniversityChulalongkorn University2018-10-192018-10-192013-01-0117th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol.1, (2013), 494-4962-s2.0-84907302434https://repository.li.mahidol.ac.th/handle/20.500.14594/31492In this work, automatic pi I changing system on lab-on-paper devices for enzyme-linked immunosorbent (ELISA) has been developed in order to improve their sensitivity and make the technique much more applicable to real samples. The most widely used enzyme in immunoassay are alkaline phosphatase (ALP) and peroxidase. ALP-conjugated secondary detection with BCIP/NBT substrate provides high sensitivity, however, the enzymatic reaction require alkaline medium. By automatic pH changing system, the immunoreaction is allowed to undergo first in the test zone at the neutral pH. Subsequently, enzymatic reaction occurs at the alkaline pH due to switching of pH from neutral to alkaline. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved.Mahidol UniversityChemical EngineeringConference PaperSCOPUS