Pranee WinichagoonJiraporn KownkonPathai YenchitsomanusVaraporn ThonglairoamNopadol SiritanaratkulSuthat FucharoenMahidol University2018-06-142018-06-141989-07-01Human Genetics. Vol.82, No.4 (1989), 389-39014321203034067172-s2.0-0024340685https://repository.li.mahidol.ac.th/handle/20.500.14594/15709Enzymatic DNA amplification and polyacrylamide gel electrophoresis, which demonstrate different sizes of DNA fragments, were used to detect the common mutations causing β-thalassemia and hemoglobin (Hb) E in Thai people. The 4-bp deletion at codons 41 and 42 can be detected directly by polyacrylamide gel electrophoresis and ethidium bromide staining. Whereas the nonsense mutations at codon 17 (AAG →TAG) and Hb E (GAG→AAG at codon 26) were detected after digestion of the amplified DNA with the enzymes MaeI and MnlI, respectively. © 1989 Springer-Verlag.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1007/BF00274004