Phattara orn HavanapanSuparat TaengchaiyaphumAtchara PaemaneeNuanwan PhungthanomSittiruk RoytrakulKallaya SritunyalucksanaChartchai KrittanaiThailand National Center for Genetic Engineering and BiotechnologyInstitute of Molecular Biosciences, Mahidol UniversityThailand National Science and Technology Development Agency2022-08-042022-08-042021-07-01Fish and Shellfish Immunology. Vol.114, (2021), 36-4810959947105046482-s2.0-85104417340https://repository.li.mahidol.ac.th/handle/20.500.14594/75635By using immunohistochemistry detection, yellow head virus (YHV) was found to replicate in granule-containing hemocytes including semi-granular hemocytes (SGC) and granular hemocytes (GC) during the early phase (24 h post injection) of YHV-infected shrimp. Higher signal of YHV infection was found in GC more than in SGC. Comparative phosphoproteomic profiles between YHV-infected and non-infected GC reveal a number of phosphoproteins with different expression levels. The phosphoprotein spot with later on identified as caspase-3 in YHV-infected GC is most interesting. Blocking caspase-3 function using a specific inhibitor (Ac-DEVD-CMK) demonstrated high replication of YHV and consequently, high shrimp mortality. The immunohistochemistry results confirmed the high viral load in shrimp that caspase-3 activity was blocked. Caspase-3 is regulated through a variety of posttranslational modifications, including phosphorylation. Analysis of phosphorylation sites of shrimp caspase-3 revealed phosphorylation sites at serine residue. Taken together, caspase-3 is a hemocytic protein isolated from shrimp granular hemocytes with a role in anti-YHV response and regulated through the phosphorylation process.Mahidol UniversityAgricultural and Biological SciencesEnvironmental ScienceImmunology and MicrobiologyArticleSCOPUS10.1016/j.fsi.2021.04.007