Peerapong Tua-NgamNittaya Jira-AnankulNiwat AnuwongnukrohSurachai DechkunakornPasaree LaokijcharoenMahidol UniversityFaculty of DentistryThailand National Metal and Materials Technology Center2018-12-212019-03-142018-12-212019-03-142017-05-31MATEC Web of Conferences. Vol.108, (2017)2261236X2-s2.0-85020430371https://repository.li.mahidol.ac.th/handle/20.500.14594/42295© The Authors, published by EDP Sciences, 2017. This study aimed to evaluate cytotoxicity of three commercial brands of orthodontic elastomeric ligatures. Materials and Methods: The three commercial orthodontic elastomeric ligature brands were C-brand, T-brand and U-brand from different manufacturing countries. The cytotoxic assay was performed using cell cultures (L929) which were subjected to the cell viability test with methyl-Tetrazolium (MTT). The L929 cell line was grown in 96-well tissue culture plates (1×105cells/cm3). Elastomeric ligatures (0.2 g) from each brand was placed in 1 ml of Minimum Essential Medium (MEM) at 37°C with 5% CO2at 100% humidity in an incubator for 24 hours. After incubation, the cell mitochondrial activity was evaluated by the MTT test for detecting viable cells from optical density according to ISO 10993-5: 2009 (E) test procedure. Cytotoxic effects were determined by the percentage of cell viability. Results: Microscopic investigation showed that all commercial elastomeric ligatures caused alterations in the fibroblast cell morphology from an elongated to a spherical form. The cell viability of C-brand, T-brand and U-brand were 51.10, 73.04 and 93.04%, respectively. Conclusion: T-brand and U-brand orthodontic elastomeric ligatures were non cytotoxic potential. U-brand had the greatest cell viability and C-brand had the lowest cell viability at 24 hours.Mahidol UniversityChemistryEngineeringConference PaperSCOPUS10.1051/matecconf/201710806004