Gunnaporn SuriyapholPrapat SuriyapholMeena SarikaputiSirin TheerawatanasirikulAchariya SailasutaChulalongkorn UniversityMahidol University2018-05-032018-05-032011-12-01Thai Journal of Veterinary Medicine. Vol.41, No.4 (2011), 509-517012564912-s2.0-84861906174https://repository.li.mahidol.ac.th/handle/20.500.14594/12894As a cornified envelope protein, filaggrin (FLG) is involved in the formation and support of the skin barrier. FLG single nucleotide polymorphism (SNP) at the position 64,297,022 (rs22588227) on canine chromosome 17 has initially been described as being associated with canine atopic dermatitis (CAD) in Labrador Retrievers in UK. In this study, we have examined whether the mentioned FLG SNP is associated with a susceptibility to CAD in small breed dogs, comprising 21 Poodles, 17 Shih tzus and 3 Pugs. Twelve of these subjects were dogs with atopy and were assigned to the experiment group and the remaining 39 samples were healthy controls. The results showed no difference of the allele frequencies at the above-mentioned position between dogs with atopic dermatitis and the controls. However, this study assessed a naturally observed sequence diversity of FLG in the dog, identifying 13 new SNPs within canine FLG and a novel repeated sequence of FLG which had not appeared in dog genome databases. Allele frequencies demonstrated that 2 of the 13 novel observed polymorphisms at the locations 64,297,000 (p = 0.041, odds ratio = 3.920) and 64,297,126 (p = 0.043, odds ratio = 3.706) were plausibly associated with a susceptibility to CAD. The effect of all SNPs was dependent on one another with a strong linkage disequilibrium (D'≥0.89) in one haplotype block (frequencies ≥2%). This study suggests a role of FLG polymorphisms in CAD and also demonstrates the successful attempts to identify another unique fragment of the repeated FLG sequence and the novel SNPs. However, since small population was included in this study, the study should be repeated with a larger population.Mahidol UniversityVeterinaryArticleSCOPUS