Prakongboon SungkasubunSurachate SiripongsakunKunlayanee AkkarachinorateSirachat VidhyarkornAkeanong WorakitsitisatornThaniya SricharunratSutida SingharuksaRawisak ChanwatChairat BunchaliewSirima CharoenphattharaphesatRuechuta MolekManeenop YimyaemGaidganok SornsamdangKamonwan SoonklangKasiruck WittayasakChirayu U. AuewarakulChulabhorn MahidolChulabhorn Graduate InstituteBan Luang HospitalMahidol UniversityNational Cancer Institute ThailandChulabhorn Research Institute2018-12-112019-03-142018-12-112019-03-142016-06-02BMC Cancer. Vol.16, No.1 (2016)147124072-s2.0-84978062499https://repository.li.mahidol.ac.th/handle/20.500.14594/42990© 2016 The Author(s). Background: Thailand has a high incidence of cholangiocarcinoma (CCA), particularly in the north and northeastern regions. Most CCA patients come at a late, unresectable stage and presently no optimal screening test for CCA has been established. We determined the prevalence of CCA in a remote northern village and explored if screening could lead to early detection and survival benefits. Methods: A 5-year population-based study was started in October, 2011 for consented Thai individuals, aged 30-60 years. The screening program comprised blood testing, stool examination and serial ultrasonography every 6 months. Results: During the first 3 years, 4,225 eligible individuals were enrolled. CCA was detected in 32 patients, with a mean age of 51.9 years (41-62 years), and 21/32 cases were at a curative resectable stage. The prevalence rate of CCA was 165.7 per 100,000 and one- and two-year incidence rate was 236.7/100,000 and 520.7/100,000, respectively. One- and 2-year overall survival rates of CCA patients were 90.9 and 61.5 %, respectively. Prognosis was better in resectable cases with 100 % 1-year and 77.8 % 2-year survival rates. Interestingly, premalignant pathological lesions (stage 0) were identified in 11 cases with 100 % 3-year survival rate. Serum biomarkers and alkaline phosphatase were not sufficient to detect early-stage disease. In 22 patients, stool samples were positive for Opistorchis viverrini, based on polymerase chain reaction. Conclusion: Detection of premalignant lesions and early-stage resectable CCA by ultrasonography resulted in improved clinical outcome. Ultrasonography should be offered as a first screening tool for CCA in an endemic area until other useful biological markers become available.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1186/s12885-016-2390-2