Natpapas WiriyachaipornWeerakanya ManeeprakornChayachon ApiwatTararaj DharakulThailand National Science and Technology Development AgencyMahidol University2018-11-232018-11-232015-01-01Microchimica Acta. Vol.182, No.1-2 (2015), 85-9314365073002636722-s2.0-84927519075https://repository.li.mahidol.ac.th/handle/20.500.14594/35775© Springer-Verlag Wien 2014. We report on a highly sensitive lateral flow immunoassay (LFIA) for influenza A which serves as a model antigen. Gold nanoparticles conjugated to monoclonal antibodies specific to the two most abundant influenza A proteins, nucleoprotein and matrix protein, were used as detector probes. Using this approach, the nucleoprotein and matrix protein in the virion were detected simultaneously. The signal was further amplified via a signal amplification strategy that is making use of two-layered nanogold in combination with a double-targeted detection format. Under optimized conditions, the system is capable of detecting influenza A antigens in infected cells at levels as low as 47 TCID50·mL−1(50 % tissue culture infectious dose) within 15 min. Compared to the conventional LFIA based on single-targeted detection, the detection capability of this system is better by a factor of 8 without requiring additional steps or instruments. In addition to its simplicity and rapidity, this LFIA also can detect the target analyte in even complex biological matrix. This proofof- principle of a dual-layered and double-targeted nanogoldbased LFIA is deemed to be useful for developing single-step, rapid, and sensitive tests for screening and diagnosis.Mahidol UniversityChemistryArticleSCOPUS10.1007/s00604-014-1303-9