Nopporn SawatjuiTeerasak DamrongrungruangWilairat LeeanansaksiriPatcharee JearanaikoonSuradej HongengTemduang LimpaiboonKhon Kaen UniversitySuranaree University of TechnologyMahidol University2018-11-232018-11-232015-07-01Materials Science and Engineering C. Vol.52, (2015), 90-96092849312-s2.0-84925813555https://repository.li.mahidol.ac.th/handle/20.500.14594/35905© 2015 Elsevier B.V. All rights reserved. Tissue engineering is becoming promising for cartilage repair due to the limited self-repair capacity of cartilage tissue. We previously fabricated and characterized a three-dimensional silk fibroin/gelatin-chondroitin sulfate-hyaluronic acid (SF-GCH) scaffold and showed that it could promote proliferation of human bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to evaluate its biological performance as a new biomimetic material for chondrogenic induction of BM-MSCs in comparison to an SF scaffold and conventional pellet culture. We found that the SF-GCH scaffold significantly enhanced the proliferation and chondrogenic differentiation of BM-MSCs compared to the SF scaffold and pellet culture in which the production of sulfated glycoaminoglycan was increased in concordance with the up-regulation of chondrogenic-specific gene markers. Our findings indicate the significant role of SF-GCH by providing a supportive structure and the mimetic cartilage environment for chondrogenesis which enables cartilage regeneration. Thus, our fabricated SF-GCH scaffold may serve as a potential biomimetic material for cartilage tissue engineering.Mahidol UniversityEngineeringMaterials ScienceMedicineArticleSCOPUS10.1016/j.msec.2015.03.043