Pinprapha SribenjaratNuananong JirakanjanakitKalyanee JirasripongpunSilpakorn UniversityMahidol University2020-08-252020-08-252020-01-01Science, Engineering and Health Studies. Vol.14, No.1 (2020), 22-31263000872-s2.0-85087468274https://repository.li.mahidol.ac.th/handle/20.500.14594/58427© 2020 Science, Engineering and Health Studies. All rights reserved. Stapinlococcus anreus is an important human pathogen. Its drug resistance strains could result in hospital-acquired infections, hi recent years, the use of nanoparticles has become a new approach to combat antibiotic-resistant bacterial strains. In the present study, green synthesis of selenium nanoparticles (SeNPs) was conducted using garlic (Allium sativum) extract (G). Formation of SeNP-G was characterized by red brick solution with maximum absorbance in the range of 270-280 nm. Particles formed at 4 h (SeNP-G4) and 72 h (SeNP-G72) of incubation were spherical in shape with size range within 21-40 nm and 41-50 nm, respectively. Zeta potential analysis of the particles was at -23.8 mV and -24.2 mV. respectively. These SeNP-G exhibited higher antibacterial activity than garlic extract in a dose-dependent manner by agar diffusion method and broth dilution assay. However, no significant difference in antibacterial capability was observed between these two particle sizes. SeNP-G72 was less cytotoxic to normal human MRC-5 cell than SeNP-G4 and sodium selenite. Thus, green nanotechnology could be used to reduce toxicity of sodium selenite and enhance antibacterial activity of garlic extract by using SeNP-G as a potential antibacterial agent against S. aureus.Mahidol UniversityMultidisciplinaryArticleSCOPUS