Patchima SithisarnLukkana SuksanpaisanChutima ThepparitDuncan R. SmithMahidol University2018-07-242018-07-242003-12-01Journal of Medical Virology. Vol.71, No.4 (2003), 532-539014666152-s2.0-0142244382https://repository.li.mahidol.ac.th/handle/20.500.14594/20858The dengue virus consists of four antigenically related but distinct viruses, termed Dengue virus 1-4 (DEN 1-4). We have established that the dengue virus loses infectivity over time in solution in an exponentially declining manner. The four strains examined (one from each serotype) have half-lives that range from 2.5 to 7.5 hr in defined medium. The half-life is temperature and pH-dependent and is affected by the nature of the host cell in which it is produced, but is not dependent upon the presence of either Mg2+ ions or chelating agents. Electron microscopy (EM) of solutions of the dengue virus show almost complete virus aggregation after 24 hr at room temperature, while RT-PCR shows an intact RNA genome. These results show that the solution environment of the dengue virus is an important determinant of dengue virus infectivity. © 2003 Wiley-Liss, Inc.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1002/jmv.10520