Sainiya SamalaSompong Te-ChatoSureerat YenchonKanchit ThammasiriRajabhat UniversityPrince of Songkla UniversityMahidol University2018-11-092018-11-092014-01-01ScienceAsia. Vol.40, No.6 (2014), 379-383151318742-s2.0-84923295862https://repository.li.mahidol.ac.th/handle/123456789/34981Viability of Grammatophyllum speciosum seeds was evaluated by a TTC (2, 3, 5-triphenyl tetrazolium chloride) test. The seeds were then asymbiotically germinated on different agar media under aseptic conditions. The results showed that keeping the seeds in the dark at 30 °C for 1 day gave 99% of red staining embryos (referred to as viable). After sowing the seeds on half-strength Gamborg's B5 medium supplemented with 0.1% activated charcoal (AC) for 30 days, germination of those seeds was observed. Half-strength MS medium (1/2 MS) supplemented with 1 mg/l 6-benzylaminopurine in combination with 0.5 mg/l alpha;-naphthalene acetic acid, 15% coconut water, 0.2% AC, and 0.7% agar gave the highest percentage of protocorm-like body (PLB) induction and proliferation. The PLBs could be regenerated into complete plantlets with vigorous shoot and root formation on 1/2 MS medium without any plant growth regulators. The survival rate of plantlets was 70% after hardening off in the greenhouse for 1 month.Mahidol UniversityMultidisciplinaryArticleSCOPUS10.2306/scienceasia1513-1874.2014.40.379