T. RojanarataD. IsarangkulS. WiyakruttaV. MeevootisomJ. M. WoodleyUCLMahidol UniversityThe Institute of Science and Technology for Research and Development, Mahidol University2018-07-242018-07-242004-05-01Biocatalysis and Biotransformation. Vol.22, No.3 (2004), 195-201102424222-s2.0-4844219951https://repository.li.mahidol.ac.th/handle/123456789/21194An isolated and immobilised aminotransferase cloned from Pseudomonas stutzeri ST-201 into Escherichia coli was used to synthesise D-phenylglycine. The reaction was characterised by an unfavourable equilibrium constant and substrate inhibition. The use of a controlled-release system via the use of Amberlite (IRA 400)-adsorbed benzoylformate proved a useful technique to circumvent these issues. This resulted in a four-fold improvement in product concentration achievable to yield a final D-phenylglycine concentration of 10.25 mg/ml. © 2004 Taylor & Francis Ltd.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringArticleSCOPUS10.1080/10242420410001727355