Takuya YamaoYuki EshitaYuki KiharaTomomitsu SathoMakoto KurodaTsuyoshi SekizukaMiho NishimuraKouji SakaiShumpei WatanabeHiroomi AkashiYupha RongsriyamNarumon KomalamisraRaweewan SrisawatTakeshi MiyataAkira SakataMasato HosokawaManabu NakashimaNobuhiro KashigeFumio MiakeShuetsu FukushiMina NakauchiMasayuki SaijoIchiro KuraneShigeru MorikawaTetsuya MizutaniFukuoka UniversityOita UniversityNational Institute of Infectious DiseasesGraduate School of Agricultural and Life Sciences The University of TokyoMahidol University2018-09-132018-09-132009-01-01Archives of Virology. Vol.154, No.1 (2009), 153-158030486082-s2.0-58149326741https://repository.li.mahidol.ac.th/handle/123456789/27747In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae. © 2008 Springer-Verlag.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1007/s00705-008-0285-5