Sunanta NabuRatana LawungPatcharee Isarankura-Na-AyudhyaSittiruk RoytrakulSupamas DolprasitSineenart SengyeeChartchalerm Isarankura-Na-AyudhyaVirapong PrachayasittikulMahidol UniversityThammasat UniversityThailand National Center for Genetic Engineering and Biotechnology2018-12-212019-03-142018-12-212019-03-142017-11-08EXCLI Journal. Vol.16, (2017), 1207-1229161121562-s2.0-85038413281https://repository.li.mahidol.ac.th/handle/123456789/41384© 2017, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved. Neisseria gonorrhoeae strains displaying reduced susceptibility and resistance to extended-spectrum cephalosporins (ESCs) are major public health concerns. Although resistance mechanisms of ESCs have extensively been studied, the proteome-wide investigation on the biological response to the antibiotic stress is still limited. Herein, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS analysis was applied to investigate the global protein expression under ESC stresses of ESC-susceptible and ESCreduced susceptible N. gonorrhoeae strains. Upon exposure to ceftriaxone, 14 and 21 proteins of ESCsusceptible and ESC-reduced susceptible strains, respectively, were shown to be differentially expressed. In the meanwhile, differential expressions of 13 and 17 proteins were detected under cefixime stress for ESCsusceptible and ESC-reduced susceptible strains, respectively. ESC antibiotics have been proven to trigger the expression of several proteins implicated in a variety of biological functions including transport system, energy metabolism, stress response and pathogenic virulence factors. Interestingly, macrophage infectivity potentiators (Ng-MIP) showed increased expression for ESC-reduced susceptible strain under ESC stress. The altered expression of Ng-MIP was found to be a unique response to ESC stresses. Our finding proposes a broad view on proteomic changes in N. gonorrhoeae in response to ESC antibiotics that provides further insights into the gonococcal antimicrobial resistance and physiological adaptation mechanism.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.17179/excli2017-832