Rachanee UdomsangpetchSangdao SomsriTasanee PanichakulKesinee ChotivanichJeeraphat SirichaisinthopZhaoqing YangLiwang CuiJetsumon SattabongkotMahidol UniversityThailand Ministry of Public HealthPennsylvania State UniversityArmed Forces Research Institute of Medical Sciences, Thailand2018-08-242018-08-242007-03-01Parasitology International. Vol.56, No.1 (2007), 65-69138357692-s2.0-33847158906https://repository.li.mahidol.ac.th/handle/123456789/24574Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology. © 2006 Elsevier Ireland Ltd. All rights reserved.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.parint.2006.12.005