M. Isabel García-LaordenArie J. HoogendijkMaryse A. WiewelLonneke A. van VughtMarcus J. SchultzNiels BovenschenAlex F. de VosTom van der PollCentro de Investigación Biomédica en Red de Enfermedades RespiratoriasUniversity Medical Center UtrechtHospital de Gran Canaria Dr. NegrinMahidol UniversityNuffield Department of MedicineAmsterdam UMC - University of Amsterdam2022-08-042022-08-042021-08-01Clinical and Experimental Immunology. Vol.205, No.2 (2021), 222-23113652249000991042-s2.0-85106702926https://repository.li.mahidol.ac.th/handle/20.500.14594/77251Sepsis is a complex syndrome related to an infection-induced exaggerated inflammatory response, which is associated with a high mortality. Granzymes (Gzm) are proteases mainly found in cytotoxic lymphocytes that not only have a role in target cell death, but also as mediators of infection and inflammation. In this study we sought to analyse the intracellular expression of GzmA, B, M and K by flow cytometry in diverse blood lymphocyte populations from 22 sepsis patients, 12 non-infected intensive care unit (ICU) patients and 32 healthy controls. Additionally, we measured GzmA and B plasma levels. Both groups of patients presented decreased percentage of natural killer (NK) cells expressing GzmA, B and M relative to healthy controls, while sepsis patients showed an increased proportion of CD8+ T cells expressing GzmB compared to controls. Expression of GzmK remained relatively unaltered between groups. Extracellular levels of GzmB were increased in non-infected ICU patients relative to sepsis patients and healthy controls. Our results show differential alterations in intracellular expression of Gzm in sepsis patients and non-infected critically ill patients compared to healthy individuals depending on the lymphocyte population and on the Gzm.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1111/cei.13601