Thongchai KaewphinitSomchai SantiwatanakulPronpan JaratsingKosum ChansiriNarong ArunrutWansika KiatpathomchaiSrinakharinwirot UniversityThailand National Center for Genetic Engineering and BiotechnologyMahidol University2018-05-032018-05-032011-12-01BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), 86-882-s2.0-84860449445https://repository.li.mahidol.ac.th/handle/20.500.14594/11882Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here a LAMP method was combined with a chromatographic lateral-flow dipstick (LFD) to detect IS6110 gene of M. tuberculosis rapidly and specifically. The reaction was optimized at 65°C for 90 min and amplified DNA hybridized to an FITC-labeled oligonucleotide probe for 5 min was detected at LFD test line 5 min after application. Excluding for the step of DNA extraction, test results could be generated within 1 h 40 min. In addition to advantages of short assay time, confirmation of amplicon identity by hybridization and elimination of electrophoresis with carcinogenic ethidium bromide, the LAMP-LFD was more sensitive than an existing PCR assay for detection of M. tuberculosis. © 2011 IEEE.Mahidol UniversityEngineeringConference PaperSCOPUS10.1109/BMEiCon.2012.6172024