Wanna SirimanapongKim D. ThompsonEi Lin OoiMichaël BekaertBertrand ColletJohn B. TaggartJames E. BronDarren M. GreenAndrew P. ShinnAlexandra AdamsMichael J. LeaverUniversity of StirlingMahidol UniversityMoredun Research InstituteNovus International, VietnamMarine ScotlandFish Vet Group, Thailand2018-11-232018-11-232015-11-01Fish and Shellfish Immunology. Vol.47, No.1 (2015), 595-60510959947105046482-s2.0-84944075394https://repository.li.mahidol.ac.th/handle/123456789/35083© 2015 Elsevier Ltd. Pangasianodon hypophthalmus (striped catfish) is an important aquaculture species and intensification of farming has increased disease problems, particularly Edwardsiella ictaluri. The effects of feeding β-glucans on immune gene expression and resistance to E. ictaluri in P. hypophthalmus were explored. Fish were fed 0.1% fungal-derived β-glucan or 0.1% commercial yeast-derived β-glucan or a basal control diet without glucan. After 14 days of feeding, the mRNA expression of immune genes (transferrin, C-reactive protein, precerebellin-like protein, Complement C3 and factor B, 2a MHC class II and interleukin-1beta) in liver, kidney and spleen were determined. Following this fish from each of the three diet treatment groups were infected with E. ictaluri and further gene expression measured 24 h post-infection (h.p.i.), while the remaining fish were monitored over 2 weeks for mortalities. Cumulative percentage mortality at 14 days post-infection (d.p.i.) was less in β-glucan fed fish compared to controls. There was no difference in gene expression between dietary groups after feeding for 14 days, but there was a clear difference between infected and uninfected fish at 24 h.p.i., and based on principal component analysis β-glucans stimulated the overall expression of immune genes in the liver, kidney and spleen at 24 h.p.i.Mahidol UniversityAgricultural and Biological SciencesEnvironmental ScienceArticleSCOPUS10.1016/j.fsi.2015.09.042