Nuanchan SamosornsukAroonluk LulitanondNuttiya SaenlaNarisara AnuntagoolSurasakdi WongratanacheewinStitaya SirisinhaKhon Kaen Regional HospitalKhon Kaen UniversityMahidol University2018-09-072018-09-071999-01-01American Journal of Tropical Medicine and Hygiene. Vol.61, No.5 (1999), 735-737000296372-s2.0-0032735043https://repository.li.mahidol.ac.th/handle/123456789/25465A monoclonal antibody (MAb)-based latex agglutination (MAb-LA) test was developed to rapidly identify Burkholderia pseudomallei in hemoculture of patients with septicemic melioidosis. The method was evaluated in a clinical situation on 396 hemocultures positive for bacterial growth, of which 75 cultures were positive for B. pseudomallei by conventional biochemical tests. The sensitivity and specificity of the MAb-LA test were 95% and 100%, respectively. The positive and negative predictive values were 100% and 99%. The method is highly reliable and suitable for rapid diagnosis of septicemic melioidosis, reducing the time normally required from a minimum of 3-4 days by conventional methods to less than 30 hr. Most of these 30 hr are involved in growing up enough bacteria to perform the MAb-LA test, which itself takes only 1 min.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.4269/ajtmh.1999.61.735