Piyawat PongpadungLitao ZhangRamaraj SathasivamKittisak YokthongwattanaNiran JuntawongJianguo LiuSangmyung UniversityNaresuan UniversityKasetsart UniversityInstitute of Oceanology Chinese Academy of SciencesMahidol University2019-08-232019-08-232018-12-01Journal of Pure and Applied Microbiology. Vol.12, No.4 (2018), 1719-17272581690X097375102-s2.0-85059464859https://repository.li.mahidol.ac.th/handle/20.500.14594/44977© 2018 The Author(s). Photosynthetic hydrogen (H 2 ) production by green algae has fascinated biologists and energy experts, due to the potential application of this process for renewable energy. In this study, H 2 photoproduction and PSII photochemical activities were investigated in Chlorella sorokiniana exposed to simultaneous nitrogen limitation and sulfur (S-) and/or phosphorus (P-) deprivation. Under S-deprivation, C. sorokiniana produced about 48.2 mL L -1 of H 2 . Moreover, simultaneous nitrogen limitation (0.7 mM NH 4 Cl) and sulfur-and/or phosphorus-deprivation significantly increased H 2 production of C. sorokiniana over that of S-deprivation alone. Maximum H 2 outputs of 77.3, 98.1 and 125.1 mL L -1 were obtained in the N-limited cultures exposed to P-deprivation (TAP-P), S-deprivation (TAP-S) and simultaneous S-and P-deprivation (TAP-S-P), respectively. The average rate of H 2 production for the N-limited culture exposed to TAP-P, TAP-S and TAP-S-P was 1.07, 1.36 and 1.50 mL L -1 h -1 , respectively. Interestingly, the H 2 inducement time in the culture subjected to simultaneous N-limitation and S-and/or P-deprivation was much shorter than that of traditional S-deprivation. The photosynthetic inhibitors, 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU) and 2, 5-dibromo-3-methyl-6-isopropylp-benzoquinone (DBMIB) repressed H 2 production in TAP-S-P (0.7 mM NH 4 Cl) medium by 68.04% and 98.65%, respectively. The conditions of simultaneous N-limitation, S-and P-deprivation provided another efficient method for inducing H 2 production in C. sorokiniana. In addition, we also found that two-thirds of the required electrons were generated from the splitting of H 2 O in PSII and that the remaining one-third possibly came from some other substrate catabolism.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.22207/JPAM.12.4.04