S. L. MoseleyP. EcheverriaJ. SeriwatanaC. TirapatW. ChaicumpaT. SakuldaipearaS. FalkowStanford UniversityMahidol University2018-10-122018-10-121982-01-01Journal of Infectious Diseases. Vol.145, No.6 (1982), 863-86915376613002218992-s2.0-0019977701https://repository.li.mahidol.ac.th/handle/20.500.14594/30334The applicability of examining clinical specimens with a DNA hybridization technique for genes encoding enterotoxins was examined using enterotoxigenic Escherichia coli (ETEC) that produced both heat-labile toxin (L T) and heat-stable toxin (ST) (24 isolates), ETEC that produced LT only (17 isolates), and ETEC that produced ST only (22 isolates) from Thailand. ETEC was identified with the Y-l adrenal cell and suckling mouse assays. All were homologous with radio labeled fragments of DNA encoding L T or ST of porcine origin (ST-P) or of human origin (ST-H). Strains of ETEC that produced ST only from rural Thailand were homologous with the ST-H probe only, whereas strains isolated in Bangkok were homologous with the ST-H probe, the ST-P probe, or both probes. The hybridization technique detected ETEC in all stool samples of patients with diarrhea from whom ETEC was isolated and in ETEC-inoculated water containing other species of bacteria. The DNA hybridization assay is useful for characterizing and identifying environmental sources of ETEC. © 1982 by the University of Chicago.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1093/infdis/145.6.863