Rungnapa VorrapakdeeSorada KanokpanontJuthamas RatanavarapornSaranatra WaikakulChris CharoenlapSiriporn DamrongsakkulChulalongkorn UniversityMahidol University2018-10-192018-10-192013-03-01Journal of Materials Science: Materials in Medicine. Vol.24, No.3 (2013), 735-74415734838095745302-s2.0-84876419630https://repository.li.mahidol.ac.th/handle/20.500.14594/31358The modification of human cancellous bone (hBONE) with silk fibroin/gelatin (SF/G) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccini-mide (NHS) crosslinking was established. The SF/G solutions at a weight ratio of 50/50 and the solution concentrations of 1, 2, and 4 wt % were studied. SF/G sub-matrix was formed on the surface and inside pore structure of hBONE. All hBONE scaffolds modified with SF/G showed smaller pore sizes, less porosity, and slightly lower compressive modulus than unmodified hBONE. SF/G sub-matrix was gradually biodegraded in collagenase solution along 4 days. The hBONE scaffolds modified with SF/G, particularly at 2 and 4 wt % solution concentrations, promoted attachment, proliferation, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSC), comparing to the original hBONE. The highest cell number, ALP activity and calcium production were observed for MSC cultured on the hBONE scaffolds modified with 4 wt % SF/G. The mineralization was also remarkably induced in the cases of modified hBONE scaffolds as observed from the deposited calcium phosphate by EDS. The modification of hBONE with SF/G was, therefore, the promising method to enhance the osteoconductive potential of human bone graft for bone tissue engineering. © 2012 Springer Science+Business Media New York.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringEngineeringMaterials ScienceArticleSCOPUS10.1007/s10856-012-4830-0