Shang YaoZimam MahmudNikoleta SachiniSathid AimjongjunPaula Saavedra-GarcíaEric W.F. LamImperial College LondonMahidol UniversityInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology-HellasPanepistimio Kritis2020-01-272020-01-272019-01-01Methods in Molecular Biology. Vol.1890, (2019), 77-90106437452-s2.0-85056409569https://repository.li.mahidol.ac.th/handle/20.500.14594/50382© 2019, Springer Science+Business Media, LLC, part of Springer Nature. FOXO3 is a tumor suppressor that orchestrates the expression of genes that regulate cell cycle progression, apoptosis, metabolism, oxidative stress, and other important cellular processes. Its inactivation is closely associated with tumorigenesis and cancer progression. On the other hand, sirtuin proteins have been demonstrated to be able to deacetylate, thus causing FOXO3 inactivation at the posttranslational level. Therefore, targeting sirtuin proteins renders new avenues for breast cancer treatment. Here, we describe three procedures for studying FOXO3 posttranslational modifications controlled by sirtuin proteins in cancer cells.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChapterSCOPUS10.1007/978-1-4939-8900-3_7