Boy M. BachtiarChatchawan SrisawatRetno Pudji RahayuRetno D. SoedjodonoSilvia Arin PrabandariEndang W. BachtiarSiriraj HospitalUniversitas AirlanggaIPB UniversityUniversitas Indonesia2022-08-042022-08-042021-01-01European Journal of Dentistry. (2021)13057464130574562-s2.0-85118529457https://repository.li.mahidol.ac.th/handle/123456789/76818Objective ?This study aimed to validate the use of Ca-apt-1, an RNA aptamer, that we generated previously as a probe for immunostaining of Candida albicans in rat tongue paraffin-fixed tissue sections Material and Methods ?The performance of Ca-apt-1 as a detector molecule was compared with that of anti- C. albicans polyclonal antibody (PcAb), which was used as a positive control. Immunostaining images were visualized by light microscopy and were analyzed by using ImageJ software. Results ?Microscopic results demonstrated that Ca-apt-1 specifically recognized and immunostained C. albicans cells of rat tongue candidiasis, with a specificity comparable to that of PcAb. ImageJ analysis showed that the area (pixels) detected by Ca-apt-1 was wider than that detected by the antibody. This indicates that the binding affinity of Ca-apt-1 toward C. albicans was better than that of PcAb on paraffin-embedded tissues. Conclusion ?This study demonstrates that Ca-apt-1 can be used as a probe for immunostaining of fixed tissue sections for oral candidiasis diagnosis.Mahidol UniversityDentistryArticleSCOPUS10.1055/s-0041-1735794