Nima Wangdi GyeltshenSungsit Sungvornyothinสังสิทธิ์ สังวรโยธินSupatra Thongrungkiatสุพัตรา ทองรุ่งเกียรติJean Pierre DujardinMahidol University. Faculty of Tropical Medicine. Department of Medical Entomology2015-07-062021-08-172015-07-062021-08-172015-07-062014https://repository.li.mahidol.ac.th/handle/123456789/63167Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 255.A double layer net (DLN) for sampling of malaria vectors was developed to compare the sampling efficacy of this method against the conventional method of human landing catch (HLC) under semi-field settings in a screen-walled corridor using laboratory reared Anopheles dirus mosquitoes. Two different colours of outer nets were also used, namely, black and white, to compare the sampling sensitivity between two different coloured materials. The results indicated that white DLN sampled more mosquitoes than the black DLN, p-value=0.015, 95% C.I.: (-2.64,-0.33). A total of 26 replicate nights with 1300 Anopheles dirus mosquitoes were used to conduct competitive comparison between the DLN and HLC, and found that the double layer net caught 7.6 ± 1.1% and human landing catch caught 15.9±2% of the released mosquitoes, respectively and the proportion of collection by double layer net against human landing catch was 0.625 ± 0.12. The result showed that the collection ratio between DLN and HLC was 1:2.09. Different genera of mosquito species showed lower collection of mosquitoes by DLN compared to HLC with the ratio of 1:1.94 for Aedes aegypti and 1:1.56 for Aedes albopictus but the comparison between the black and white DLN showed that there was no statistical significance difference on the number of Aedes aegypti sampled by the two types of nets, p=0.86, whereas, in the case of Aedes albopictus, black DLN was fond slightly more efficient that white DLN, p=0.006, 95% C.I.: (0.82, 4.36). The double layer net does not expose people to potentially infectious mosquito bites and it is designed to catch host-seeking mosquitoes only. Although the sensitivity is half that of conventional method, yet, these traps could be further improved upon by conducting field trails and its sensitivities further increased.engMahidol UniversityAnopheline MosquitoesAnopheles dirusDouble layer netHuman landing catchProceeding Poster