Siriporn ChamniansawatSukumal ChongthammakunMahidol University2018-09-132018-09-132009-03-13Neuroscience Letters. Vol.452, No.2 (2009), 130-135030439402-s2.0-60249089939https://repository.li.mahidol.ac.th/handle/20.500.14594/28304Activity-regulated cytoskeleton associated protein (Arc) is known to be induced by synaptic plasticity following memory consolidation. Since estrogen has been shown to play an important role in synaptogenesis, a key aspect of the synaptic plasticity, we aimed to study the effects of estrogen on Arc expression in SH-SY5Y human neuroblastoma cells. Using quantitative real-time PCR, Western blot, and confocal immunocytochemistry techniques we found that estrogen markedly increased Arc mRNA and protein expression in SH-SY5Y cells. Estrogen-activated Arc expression was mediated via mitogen-activated protein kinase (MAPK) and phosphoinositide-3 kinase (PI-3K), but not protein kinase C (PKC) and Rho-associated kinase (ROCK), and in the estrogen receptor (ER)-dependent manner. Estrogen also significantly upregulated the dendritic spine scaffolding protein, postsynaptic density-95 (PSD-95), as well as expression of the presynaptic vesicle protein, synaptophysin. Our findings demonstrate the possible mechanisms of estrogen-induced synaptic plasticity, as well as memory consolidation. © 2009 Elsevier Ireland Ltd. All rights reserved.Mahidol UniversityNeuroscienceArticleSCOPUS10.1016/j.neulet.2009.01.010