Pewpan M. IntapanChakrit HirunpetcharatChurairat KularbkaewWiboonchai YutanawiboonchaiPenchom JanwanWanchai MaleewongKhon Kaen UniversityMahidol University2018-10-192018-10-192013-12-01Korean Journal of Parasitology. Vol.51, No.6 (2013), 637-64417380006002340012-s2.0-84893281596https://repository.li.mahidol.ac.th/handle/20.500.14594/31825This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response. © 2013, Korean Society for Parasitology and Tropical Medicine.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.3347/kjp.2013.51.6.637