Sontisiri P.Promrug D.Srichaimoon L.Arthan D.Pimtong W.Thongyoo P.Mahidol University2025-04-172025-04-172025-06-15Bioorganic Chemistry Vol.160 (2025)00452068https://repository.li.mahidol.ac.th/handle/123456789/109583A new photoactivatable “TURN-ON” fluorescent probe for H₂S detection was designed based on a tribromocoumarin scaffold, and successfully implemented through O-sulfonylation between a 3,6,8-tribromo-7-hydroxy-4-methylcoumarin (TBC) fluorophore and a dabsyl quencher. The H₂S-responsive strategy of the dabsyltribromocoumarin (Dab-TBC) probe is initiated via light-induced thiolysis of a sulfonate ester. This probe demonstrated excellent sensitivity and great stability towards H₂S detection under UV light irradiation, with no interference from other analytes, achieving a detection limit (LoD) of 1.61 μM. Importantly, Dab-TBC exhibited superb membrane permeability and showed great potential for visualizing H₂S levels in HeLa cells and zebrafish models, with no toxicity confirmed by in vivo toxicity studies in zebrafish embryos.Pharmacology, Toxicology and PharmaceuticsChemistryBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.bioorg.2025.1084472-s2.0-10500233270810902120