Naoya KataokaAlisa S. VangnaiThunyarat PongtharangkulToshiharu YakushiKazunobu MatsushitaYamaguchi UniversityChulalongkorn UniversityMahidol University2018-12-212019-03-142018-12-212019-03-142017-12-01Bioresource Technology. Vol.245, (2017), 1538-154118732976096085242-s2.0-85019629867https://repository.li.mahidol.ac.th/handle/20.500.14594/42130© 2017 Elsevier Ltd To expand the diversity of chemical compounds produced through microbial conversion, a platform pathway for the production of widely used industrial chemicals, 1,3-diols, was engineered in Escherichia coli. The pathway was designed by modifying the previously reported (R)-1,3-butanediol synthetic pathway to consist of pct (propionate CoA-transferase) from Megasphaera elsdenii, bktB (thiolase), phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, bld (butyraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and the endogenous alcohol dehydrogenase(s) of E. coli. The recombinant E. coli strains produced 1,3-pentanediol, 4-methyl-1,3-pentanediol, and 1,2,4-butanetriol, together with 1,3-butanediol, from mixtures of glucose and propionate, isobutyrate, and glycolate, respectively, in shake flask cultures. To the best of our knowledge, this is the first report of microbial production of 1,3-pentanediol and 4-methyl-1,3-pentanediol.Mahidol UniversityChemical EngineeringEnergyEnvironmental ScienceArticleSCOPUS10.1016/j.biortech.2017.05.082