Thichakorn JittawuttipokaSarinya BuranajitpakornMayuree FuangthongHerbert P. SchweizerPaiboon VattanaviboonSkorn MongkolsukChulabhorn Research InstituteMahidol UniversityChulabhorn Graduate InstituteColorado State University2018-09-132018-09-132009-09-01FEMS Microbiology Letters. Vol.298, No.1 (2009), 111-11715746968037810972-s2.0-68149145662https://repository.li.mahidol.ac.th/handle/123456789/27151Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome was detected at a Tn7 attachment (attTn7) site located downstream of glmS1. Furthermore, using a newly constructed vector pBBR1FLP2 containing the flipase (FLP) recombinase for site-specific excision of the sequence between the FLP recombinase target (FRT) sites, and a sacB counter selection marker, an unmarked mini-Tn7 insertion mutant was created. Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP-FRT systems also work well in Xanthomonas oryzae pv. oryzae. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1111/j.1574-6968.2009.01707.x