Jitladda TangpakdeeYasuyuki TanakaKyozo OguraTanetoshi KoyamaRapepun WititsuwannakulDhirayos WititsuwannakulKasem AsawatreratanakulTokyo University of Agriculture and TechnologyTohoku UniversityPrince of Songkla UniversityMahidol University2018-07-042018-07-041997-05-01Phytochemistry. Vol.45, No.2 (1997), 261-267003194222-s2.0-0030891340https://repository.li.mahidol.ac.th/handle/123456789/17862The isomerization of isopentenyl diphosphate (IDP) to dimethylallyl diphosphate (DMADP), and the formation of oligoprenyl diphosphates as well as polyprenyl diphosphates, catalyzed by IDP-isomerase and polyprenyl transferases, respectively, were observed on incubation of a mixture of14C-IDP and fresh bottom fraction (BF) (separated from centrifuged fresh Hevea latex) which had been preincubated at 4°overnight. The incubation era freeze-dried BF with14C-IDP gave DMADP, farnesyl diphosphate (FDP) and polyprenyl diphosphate as predominant products, while that of fresh BF gave C20- and C40-diphosphates, as well as rubber as main products. FDP was not detected in the incubation of the fresh BF, suggesting that it was consumed as a primer for the formation of rubber and polyprenols. Direct evidence was obtained to show the presence of IDP-isomerase and polyprenyl transferase activities in the BF of fresh latex.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/S0031-9422(96)00837-0