V. BoonsaengS. PanyimP. WilairatA. TassanakajonMahidol University2018-08-102018-08-101993-01-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.87, No.3 (1993), 273-27518783503003592032-s2.0-0027268678https://repository.li.mahidol.ac.th/handle/123456789/22586The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections. © 1993 Oxford University Press.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/0035-9203(93)90124-9