Pornratananont G.Tangprasittipap A.Wongborisuth C.Chumchuen S.Bhukhai K.Anurathapan U.Hongeng S.Songdej D.Mahidol University2025-06-072025-06-072025-08-01Stem Cell Research Vol.86 (2025)18735061https://repository.li.mahidol.ac.th/handle/123456789/110567The HBB gene encodes the β-globin protein, a component of adult hemoglobin A (HbA) which is responsible for the transportation of oxygen. Mutations in the HBB gene can impair β-globin synthesis and disrupt hemoglobin production. Patients who possess both a protein-reducing β-thalassemia mutation and a β<sup>E</sup> mutation in their HBB gene are affected by hemoglobin E/β-thalassemia disease. This study demonstrates the successful generation and characterization of the human pluripotent stem cell (hiPSC) line MURAi002-A derived from a patient with hemoglobin E/β<sup>0</sup>-thalassemia disease harboring the specific codon 41/42 (−CTTT) β<sup>0</sup>-thalassemia mutation through the utilization of non-integrative reprogramming episomes.Biochemistry, Genetics and Molecular BiologyGeneration of integration-free induced pluripotent stem cell (iPSC) line MURAi002-A from hemoglobin E/β-thalassemia disease patient harboring βE/β0 (CD41/42, –CTTT) compound heterozygous mutationArticleSCOPUS10.1016/j.scr.2025.1037432-s2.0-10500676289918767753