Supanat LumbikanandaThanaporn SriwantanaKrit RattanawonsakulTipparat ParakawChareerut PhruksaniyomKanyawat RattanasuwanPornpun VivithanapornCharoensri ThonabulsombatNathawut SibmoohSirada SrihirunMahidol University, Faculty of DentistryFaculty of Medicine Ramathibodi Hospital, Mahidol UniversityMahidol University2022-08-042022-08-042021-11-01Nitric Oxide - Biology and Chemistry. Vol.116, (2021), 1-610898611108986032-s2.0-85112423796https://repository.li.mahidol.ac.th/handle/123456789/75990Nitrite anion (NO2−) is a circulating nitric oxide (NO) metabolite considered an endothelial function marker. Nitrite can be produced from nitrate (NO3−) secreted from plasma into saliva. The nitrate reductase of oral bacteria converts salivary nitrate to nitrite, which is swallowed and absorbed into circulation. In this study, we aimed to examine the relevance between these species' salivary and blood levels. We collected three whole saliva samples (unstimulated, paraffin-stimulated, and post-chlorhexidine mouthwash stimulated saliva) and blood from 75 healthy volunteers. We measured the nitrite and nitrate by the chemiluminescence method. The nitrite levels in stimulated saliva and post-mouthwash stimulated saliva exhibited weak correlations with blood nitrite. There was no correlation between nitrite in unstimulated saliva with blood nitrite. The baseline platelet activity, determined as P-selectin expression, negatively correlated with nitrite in plasma and post-mouthwash stimulated saliva. The salivary nitrate in all saliva samples showed correlations with its plasma levels. We conclude that nitrite in stimulated saliva correlates with blood nitrite.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.niox.2021.08.002