Sumonmal UttayamakulSirirat LikanonsakulRujanee SunthornkachitKaroon KuntiranontSuda LouisirirotchanakulAchara ChaovavanichVipa ThiamchaiSombat TanprasertsukRuengpung SutthentMahidol UniversityThailand Ministry of Public HealthSTD and TB2018-06-212018-06-212005-09-01Journal of Virological Methods. Vol.128, No.1-2 (2005), 128-134016609342-s2.0-22144469748https://repository.li.mahidol.ac.th/handle/20.500.14594/16555The usage of dried blood spots as specimens for diagnosis and monitoring of HIV-1 infection in Thailand was evaluated. EDTA blood samples, which were collected from 100 HIV seronegative and 109 HIV seropositive individuals, were tested on dried blood spots; Whatman, Schleicher and Schuell (S&S) No. 903 and S&S IsoCode filter paper. Nucleic acid was extracted and used as a template for HIV-1 proviral DNA detection by an "in-house" multiplex PCR and a commercial Amplicor HIV-1 PCR test (Roche, version 1.0). HIV-1 RNA qualitative (QL) and quantitative (QT) detection was determined by Nucleic Acid Sequence Based Amplification (NASBA). The average DNA per blood spot recovered from Whatman and S&S IsoCode was not statistically different (p = 0.512) with a range of 218.9 ± 46.84 and 225.63 ± 88.33 μg, respectively. The concordance of HIV-1 proviral DNA detection by PCR from dried blood spots Whatman and S&S IsoCode was 94% versus 89.4% for sensitivity and 100% versus 100% for specificity. The sensitivity and specificity of HIV-1 RNA QL detection in dried blood spots was 89.7 and 97.5%, respectively. The HIV-1 RNA QT from dried blood spots showed a good correlation in paired dried blood spots and plasma with Pearson correlation, r = 0.817 (R2= 0.667, P < 0.05). The data showed that dried blood spots could be used for the diagnosis and monitoring of HIV-1 infection. © 2005 Elsevier B.V. All rights reserved.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1016/j.jviromet.2005.04.010