Kabkaew L. SukontasonTarinee ChaiwongUrai ChaisriHiromu KurahashiMichelle SanfordKom SukontasonChiang Mai UniversityUbon Rajathanee UniversityMahidol UniversityNational Institute of Infectious DiseasesTexas A and M University2018-05-032018-05-032011-10-11Journal of Parasitology Research. Vol.2011, (2011)20900031209000232-s2.0-80053464389https://repository.li.mahidol.ac.th/handle/20.500.14594/11977This work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-day-old flies and observed under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM displayed a smooth surface which is occasionally penetrated by tracheoles. TEM of the testis in the pupal stage presents the thick testis wall covering underdeveloped cells containing a variable size of an electron-dense globule. For the 3-day-old males, the testicular wall is formed by an external layer, a peritoneal sheath, a muscular layer, a basement membrane, and a follicular epithelium. Follicular epithelium presented developing spermatozoa. Regarding the 7-day-old males, development of spermatozoa is apparent, displaying nucleus, centriolar adjunct, axoneme, and mitochondrial derivatives, with the 9 + 9 + 2 microtubule pattern of axoneme. © 2011 Kabkaew L. Sukontason et al.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1155/2011/690863