Pleumchitt RojanapanthuNarong SarisutaKorakot ChaturonKrisana KraisintuMahidol UniversitySilpakorn UniversityThailand Government Pharmaceutical Organization2018-07-242018-07-242003-02-24Drug Development and Industrial Pharmacy. Vol.29, No.1 (2003), 31-37036390452-s2.0-0037276589https://repository.li.mahidol.ac.th/handle/123456789/20761The physicochemical properties of phosphatidylcholine-cholesterol liposomes containing amphotericin B and prepared by reverse-phase evaporation method were studied. Uniformly dispersed liposomal suspensions were obtained by employing 3:1 ratio (by volume) of diethyl ether to normal saline, 5 min sonication time at 7°C, and evaporation of diethyl ether at 25°C. Microscopic examination showed that the prepared liposomes were spheroids with unilamellar, oligolamellar, or multilamellar structure. The liposomes containing amphotericin B 2.0 mol% of total lipid led to the highest percentage of drug entrapment. Liposomes with maximum entrapment efficiency were obtained from using 250 μmol of total lipid. The liposomal amphotericin B possessing the highest drug entrapment efficiency (approximately 95%) with particle size range of 1307-1451 nm was the one composed of 1:1 molar ratio of phosphatidylcholine to cholesterol.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1081/DDC-120016681