Patinya SukwongSupunsa KongsengSunisa ChaicherdKrongtong YoovathawornSuchakree TubtimkunaDakrong PissuwanMahidol University2018-12-212019-03-142018-12-212019-03-142017-10-01IET Nanobiotechnology. Vol.11, No.7 (2017), 759-765175187412-s2.0-85029691841https://repository.li.mahidol.ac.th/handle/20.500.14594/41750© The Institution of Engineering and Technology 2017. Titanium dioxide nanoparticles (TiO2-NPs) have been increasingly mixed in food and daily use products. Therefore, the investigation of cytotoxic effects of TiO2-NPs is required to allay concerns of health effects related to contact with products containing TiO2-NPs. In this study, the authors demonstrated how TiO2-NPs impact on two main sub-types of immune cells that play a major role in adaptive and innate immune system. Human T-lymphocytes (Jurkat cells) and murine macrophages (RAW 264.7 cells) were used in this study. The authors results showed that cell viability of Jurkat and RAW 264.7 cells were significantly decreased, when cells were treated with TiO2-NPs at 250 and 500 μg/ml. However, the decrease of cell viability of RAW 264.7 cells was higher than that of Jurkat cells. A similar trend was also found in DNA fragmentation. An induction of reactive oxygen species was detected in both cells treated with TiO2-NPs at concentrations ≥25 μg/ml. A significant induction of tumour necrosis factor alpha (TNF-α) was found in Jurkat and RAW 264.7 cells treated with 25 μg/ml TiO2-NPs. In contrast, there was no significant induction of interleukin-6 (IL-6) in both cells that were treated with different concentrations of TiO2-NPs.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyEngineeringArticleSCOPUS10.1049/iet-nbt.2016.0205