Duval, Brea D.Elrod, Mindy G.Gee, Jay E.Narisara Chantratitaนริศรา จันทราทิตย์Sarunporn Tandhavanantศรัณย์พร ตันฑวนันท์Direk Limmathurotsakulดิเรก ลิ้มมธุรสกุลHoffmaster, Alex R.Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology.Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit.Mahidol University. Faculty of Tropical Medicine.Department of Tropical Hygiene.2014-08-282016-11-092014-08-282016-11-0920142014-08-272014-04-07Duval BD, Elrod MG, Gee JE, Chantratita N, Tandhavanant S, Limmathurotsakul D. et al. Evaluation of a latex agglutination assay for the identificationof Burkholderia pseudomallei and Burkholderia mallei. Am J Trop Med Hyg. 2014 Jun;90(6):1043-6.0002-9637 (printed)1476-1645 (electronic)https://repository.li.mahidol.ac.th/handle/20.500.14594/857Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.engMahidol UniversityBurkholderia malleiBurkholderia pseudomalleiOpen Access articleArticlePubMed Cental10.4269/ajtmh.14-0025.