Sukjee W.Thitithanyanont A.Manopwisedjaroen S.Seetaha S.Thepparit C.Sangma C.Mahidol University2023-06-182023-06-182022-05-15Materials Letters Vol.315 (2022)0167577Xhttps://repository.li.mahidol.ac.th/handle/20.500.14594/84597SARS-CoV-2 is the virus responsible for causing the global COVID-19 pandemic. Identifying the presence of this virus in the environment could potentially improve the effectiveness of disease control measures. Environmental SARS-CoV-2 monitoring may become increasingly demanded in areas where the available testing methods are ineffective. In this study, we present an electrochemical polymer composites biosensor for measuring SARS-CoV-2 whole-virus particles in the environment. The sensitized layer was prepared from molecularly imprinted polymer (MIP) composites of inactivated SARS-CoV-2. Testing demonstrated increased sensor signaling with SARS-CoV-2 specifically, while lower responses were observed to the negative controls, H5N1 influenza A virus and non-imprinted polymers (NIPs). This sensor detected SARS-CoV-2 at concentrations as low as 0.1 fM in buffer and samples prepared from reservoir water with a 3 log-scale linearity.EngineeringArticleSCOPUS10.1016/j.matlet.2022.1319732-s2.0-8512547100518734979