Preeyaporn KoedrithYeo Jin KimYounghun KimJoo Hyon KangYoung Rok SeoFaculty of Environment and Resource Studies, Mahidol UniversityKwangwoon UniversityDongguk University, SeoulInstitute of Environmental Medicine for Green Chemistry2019-08-232019-08-232018-07-01Molecular and Cellular Toxicology. Vol.14, No.3 (2018), 273-282209284671738642X2-s2.0-85060332500https://repository.li.mahidol.ac.th/handle/20.500.14594/45878© 2018, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V. Backgrounds: Trends in the use of an anticoagulant as a dispersing stabilizer are addressed. An effective approach to preparing stable nanosized titanium dioxide (nTiO 2 ) for accurate and systematic assessment of nano- toxicity has not been established. Methods: Among the dispersants tested here, it was found that sodium polyacrylate (PAA) was the most effective dispersant for nTiO 2 in culture media. Our study was the first to demonstrate that a stable PAA-dispersed nTiO 2 (nTiO 2 /PAA) suspension showed more toxic than nTiO 2 without PAA in human HaCaT keratinocytes. Results: Initially, MTT results showed that the stable nTiO 2 /PAA dispersion exhibited significantly greater cytotoxicity than nTiO 2 without PAA. In addition, the stable nTiO 2 /PAA dispersion induced markedly more oxidative stress than nTiO 2 without PAA. Importantly, the stable nTiO 2 /PAA dispersion caused DNA breakage to a greater extent than nTiO 2 without PAA. Conclusion: Our findings indicated that the anti-coagulant PAA is suitable for preparing homologous dispersed nTiO 2 under realistic physiological culture test conditions.Mahidol UniversityEnvironmental ScienceMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1007/s13273-018-0030-4