The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle

Jeerus SucharitakulChanakan TongsookDanaya PakotipraphaWillem J.H. Van BerkelPimchai ChaiyenChulalongkorn UniversityMahidol UniversityWageningen University and Research Centre2018-10-192018-10-192013-12-06Journal of Biological Chemistry. Vol.288, No.49 (2013), 35222-352361083351X002192582-s2.0-84894312047https://repository.li.mahidol.ac.th/handle/123456789/31142Background: 3-Hydroxybenzoate 6-hydroxylase (3HB6H) is a flavoprotein hydroxylase catalyzing the para-hydroxylation of 3-hydroxybenzoate. Results: The oxidative half-reaction was studied using transient kinetics. The enzyme reaction mechanism was elucidated. Conclusion: The product release and the hydroxylation limit the turnovers and the enzyme shows characteristics different from the ortho-hydroxylation enzymes. Significance: This is the first report on the oxygenation mechanism of a para-hydroxylating flavoenzyme. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyThe reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycleArticleSCOPUS10.1074/jbc.M113.515205