Jisnuson SvastiThanawat PhongsakRakrudee SarnthimaMahidol University2018-07-242018-07-242003-06-06Biochemical and Biophysical Research Communications. Vol.305, No.3 (2003), 470-4750006291X2-s2.0-0037902560https://repository.li.mahidol.ac.th/handle/123456789/20719We have compared the ability of β-glucosidases from cassava, Thai rosewood, and almond to synthesize alkyl glucosides by transglucosylating alkyl alcohols of chain length C1-C8. Cassava linamarase shows greater ability to transfer glucose from p-nitrophenyl-β-glucoside to secondary alcohol acceptors than other β-glucosidases, and is unique in being able to synthesize C4, C5, and C6tertiary alkyl β-glucosides with high yields of 94%, 82%, and 56%, respectively. Yields of alkyl glucosides could be optimized by selecting appropriate enzyme concentrations and incubation times. Cassava linamarase required pNP-glycosides as donors and could not use mono- or di-saccharides as sugar donors in alkyl glucoside synthesis. © 2003 Elsevier Science (USA). All rights reserved.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/S0006-291X(03)00793-9