Lyda OsorioNick CarterPreetam ArthurGermana BanconeSowmya GopalanSandeep K. GuptaHarald NoedlSanjay K. KocharDhanpat K. KocharSrivicha KrudsoodMarcus V. LacerdaAlejandro Llanos-CuentasRonnatrai RueangweerayutRamadurai SrinivasanMoritz TreiberJörg J. MöhrleJustin GreenUniversidad del Valle, CaliGlaxoSmithKline plc.Sri Ramachandra Medical College & Research Institute (Deemed University)Shoklo Malaria Research UnitMV Hospital and Research CentreMedizinische Universitat WienSardar Patel Medical CollegeUniversity of RajasthanMahidol UniversityFundacao de Medicina Tropical do AmazonasUniversidad Peruana Cayetano HerediaMae Sot General HospitalClinical Development2018-11-232018-11-232015-01-01American Journal of Tropical Medicine and Hygiene. Vol.92, No.1 (2015), 22-27000296372-s2.0-84920557534https://repository.li.mahidol.ac.th/handle/20.500.14594/36181Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. Accurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in 356 Plasmodium vivax-infected subjects in Brazil, Peru, Thailand, and India. In the quantitative assay, the median G6PD activity was 8.81 U/g hemoglobin (range = 0.05-20.19), with 11 (3%) subjects identified as deficient. Sensitivity of the BinaxNOW G6PD to detect deficient subjects was 54.5% (6 of 11), and specificity was 100% (345 of 345). Room temperatures inadvertently falling outside the range required to perform the rapid test (18-25°C) together with subtlety of color change and insufficient training could partially explain the low sensitivity found. Ensuring safe use of 8-aminoquinolines depends on additional development of simple, highly sensitive G6PD deficiency diagnostic tests suitable for routine use in malaria-endemic areas.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.4269/ajtmh.14-0298