Ratana LawungRungrot CherdtrakulkiatAngkana CharoenwatanachokchaiSunanta NabuWanvisa SuksalukVirapong PrachayasittikulMahidol UniversityThailand Ministry of Public Health2018-09-132018-09-132009-06-01Journal of Microbiological Methods. Vol.77, No.3 (2009), 323-325016770122-s2.0-67349175771https://repository.li.mahidol.ac.th/handle/20.500.14594/27209One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction. © 2009 Elsevier B.V. All rights reserved.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.mimet.2009.03.009