Porntip PetmitrGanokwan PongvilairatPrapon WilairatMahidol University2018-07-042018-07-041997-03-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.1 (1997), 18-21012515622-s2.0-0031087759https://repository.li.mahidol.ac.th/handle/123456789/18150A large scale technique for pure gametocyte cultures of Plasmodium falciparum was established in culture flasks by using treated erythrocytes and RPMI medium supplemented with 15% human plasma instead of serum. Pure gametocyte cultures were successfully obtained following treatment with 5% sorbitol on day 8 and 9 of cultivation This method resulted in approximately 97% reduction of asexual parasites and provided pure gametocytes in culture. The highest numbers of gametocytes were obtained from cultures starting with 2% parasitemia and 2% erythrocyte suspension. On day 12 of cultivation, approximately 35 × 106 gametocytes per 100 ml of cell suspension could be harvested.Mahidol UniversityMedicineArticleSCOPUS