Somporn SrifuengfungSusan AssanasenMalulee TuntawiroonSumonrat MeejanpetchMahidol University2018-09-242018-09-242010-08-01Asian Biomedicine. Vol.4, No.4 (2010), 631-635190574152-s2.0-77957961895https://repository.li.mahidol.ac.th/handle/123456789/28658Background: Siderophore is an iron chelator produced by microorganism. Pseudomonas aeruginosa produces two siderophores (pyoverdin and pyochelin). Desferrioxamine is a siderophore used in thalassemia patients to treat an iron overload of vital organs. Objective: Compare the ability of pyoverdin, pyochelin, and desferrioxamine for iron mobilization from ferritin. Materials and Methods: In vitro experiment, the ability of P. aeruginosa siderophores and desferrioxamine for iron mobilization from ferritin was compared by using a dialysis membrane assay at pH values of 7.4 and 6.0. Stimulation of P. aeruginosa PAO1 growth by all siderophores was studied in glucose minimum medium. Results: All three compounds were capable of iron mobilization at both pHs. At pH 6.0, the most effectiveness compound was desferrioxamine (31.6%), followed by pyoverdin (21.5%) and pyochelin (13.7%) compared on weight basis, each at 10 μg/mL. At equimolar concentration, their activities were desferrioxamine (38.5 ±1.2%), followed by pyoverdin (32.0 ±4.8%) and pyochelin (26.7 ±1.9%), respectively. Conclusion: The most effective compound in iron mobilization from ferritin was desferrioxamine, followed by pyoverdin and pyochelin respectively.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS