N. RahmahB. H. LimA. Khairul AnuarR. K. ShenoyV. KumaraswamiS. Lokman HakimP. ChotechuangK. KanjanopasC. P. RamachandranSchool of Medical Sciences - Universiti Sains MalaysiaUniversity of MalayaT.D. Medical College HospitalTuberculosis Research Centre IndiaInstitute for Medical Research Kuala LumpurMahidol UniversityThailand Ministry of Public HealthUniversiti Putra Malaysia2018-09-072018-09-072001-01-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.95, No.3 (2001), 280-284003592032-s2.0-0035348067https://repository.li.mahidol.ac.th/handle/123456789/26598An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0·300, the IgG4 ELISA demonstrated specificity rates of 95·6-100%, sensitivity rates of 96-100%, positive predictive values of 75-100% and negative predictive values of 98·9-100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/S0035-9203(01)90234-2